Making Diluted Solutions of MicroSpheres
Procedure - From liquid with ~ 10% solids
- Shake microsphere bottle to evenly disperse the particles.
- Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution).
Anti-Stick Agents
- Bovine Serum Albumin (BSA) at 20-25mg/ml in deionized water can be applied to the glass microscope slide (and coverslip) and allowed to evaporate off (to inhibit particle sticking to the glass surfaces).
- For non-biological particles, 1% polyethylene glycol can be added to the microsphere solution as an anti-stick agent.
- For biological particles the BSA application and Phosphate-Buffered Saline (PBS - 0.8% NaCl, 0.02% KCL, 0.02M PO4, pH 7.3 (percentage by weight)) can be used as anti-stick agents.
Students: If this recipe is inadequate (too many or too few particles) please revise.
