Vibrio Cholerae

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Vibrio Cholerae bacteria was obtained from the Biophysics Department. This particular sample was chosen because the individual bacteria are of similar size as the microspheres, and have flagellum (tail-like structure) which allows them to traverse the fluid in which the are living in. An image of a single vibrio cholera organism is show below:Vibrio.jpg The body is about 2-3 micrometers long and about 1 micrometers wide.

You will need assistance procuring a sample, but a basic understanding about handling the samples is important: First the bacteria will be diluted in a nutrient broth which will keep them alive. It is important to dilute the sample enough because just as the microspheres a overly concentrated sample will be impossible to work with. Additionally the