Difference between revisions of "Making Diluted Solutions of MicroSpheres"
| Line 7: | Line 7: | ||
=== Anti-Stick Agents === | === Anti-Stick Agents === | ||
| − | * Bovine Serum Albumin (BSA) at | + | * Bovine Serum Albumin (BSA) at 20-25mg/ml in deionized water can be applied to the glass microscope slide (and coverslip) and allowed to evaporate off (to inhibit particle sticking to the glass surfaces). |
| − | * For biological particles the BSA | + | * For non-biological particles, 1% polyethylene glycol can be added to the microsphere solution as an anti-stick agent. |
| + | |||
| + | * For biological particles the BSA application and Phosphate-Buffered Saline (PBS - 0.8% NaCl, 0.02% KCL, 0.02M PO4, pH 7.3 (percentage by weight)) can be used as anti-stick agents. | ||
[http://en.wikipedia.org/wiki/Phosphate_buffered_saline PBS Wikipedia] | [http://en.wikipedia.org/wiki/Phosphate_buffered_saline PBS Wikipedia] | ||
''Students: If this recipe is inadequate (too many or too few particles) please revise. '' | ''Students: If this recipe is inadequate (too many or too few particles) please revise. '' | ||
Revision as of 15:21, 24 November 2014
Procedure - From liquid with ~ 10% solids
- Shake microsphere bottle to evenly disperse the particles.
- Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution).
Anti-Stick Agents
- Bovine Serum Albumin (BSA) at 20-25mg/ml in deionized water can be applied to the glass microscope slide (and coverslip) and allowed to evaporate off (to inhibit particle sticking to the glass surfaces).
- For non-biological particles, 1% polyethylene glycol can be added to the microsphere solution as an anti-stick agent.
- For biological particles the BSA application and Phosphate-Buffered Saline (PBS - 0.8% NaCl, 0.02% KCL, 0.02M PO4, pH 7.3 (percentage by weight)) can be used as anti-stick agents.
Students: If this recipe is inadequate (too many or too few particles) please revise.
