Difference between revisions of "Optical Tweezers"
(→Optical Tweezers) |
|||
Line 145: | Line 145: | ||
<ul> | <ul> | ||
<li>[[Trapping Bacteria]]</li> | <li>[[Trapping Bacteria]]</li> | ||
− | + | <li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li> | |
− | <li>[[Asymmetric]]</li> | + | </ul> |
− | |||
</li> | </li> |
Revision as of 20:30, 23 August 2014
Contents
Optical Tweezers
Resources
- [1] Directions on how to use a QPD in an optical tweezer setup.
- [2] Here are some slide prepping instructions from Berkeley.
Our own setup
- Slide Setup
- Microscope Slide Mount
-
Stokes' Setup
- Spring 2013 Method (ramped) [3]
-
Summer 2014 Method (sinusoidal)
[4]
- Stoke's Force Calibration Video (sinusoidal)[5]
Using NI Vision Assistant
- [6] Image Acquisition/Saving Images
-
[7] How to track the microspheres in NI Vision Assistant using pattern matching.
- We actually tried both using pattern matching and brute force point and click methods. Neither worked very well, so we recommend using the QPD to get position measurements
Our calculations using Brownian Motion
Calculating Trap Forces Using Stokes' Drag Force
- [10] iPython Calculations
-
Beam Power (mW) Escape Velocity (microns/second) Trap Force (pN) 5.5 20.57 0.44 8.5 33.49 0.72 11.7 40.00 0.86 15.3 62.60 1.34 19.0 84.71 1.82 23.0 110.77 2.38 - A simple calculation would say that I need a 10 billion watt laser to achieve a 1 Newton trapping force. We should totally do that. The spheres would be so incredibly trapped.
Trapping Video
Performing Biological Measurements
- Installing Infared Laser
- Preparing bacteria to Analyze
- Current Progress and Issues