Difference between revisions of "Optical Tweezers"
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[http://labs.physics.berkeley.edu/mediawiki/index.php/Optical_Trapping#Preparing_a_Slide] | [http://labs.physics.berkeley.edu/mediawiki/index.php/Optical_Trapping#Preparing_a_Slide] | ||
| − | [[File:optical tweezer slide 1.jpg| | + | |
| − | [[File:optical tweezer slide 2.jpg]] | + | <p>[[File:optical tweezer slide 1.jpg|500px]] </p> |
| − | [[File:optical tweezer slide 3.jpg]] | + | Place lens tissue paper above and below a microscope slide. |
| − | [[File:optical tweezer slide 4.jpg]] | + | |
| − | [[File:optical tweezer slide 5.jpg]] | + | <p>[[File:optical tweezer slide 2.jpg|500px]]</p> |
| − | [[File:optical tweezer slide 6.jpg]] | + | To clean the slide, drop a small amount of isopropyl alcohol on top of the top piece of lens tissue. |
| − | [[File:optical tweezer slide 7.jpg]] | + | |
| − | [[File:optical tweezer slide 8.jpg]] | + | <p>[[File:optical tweezer slide 3.jpg|500px]]</p> |
| − | [[File:optical tweezer slide 9.jpg]] | + | Slide the lens tissue across the microscope slide, making sure that the alcohol wipes across the entire slide. You may need something to hold the slide in place to keep it from... well, sliding. |
| − | [[File:optical tweezer slide 10.jpg]] | + | |
| − | [[File:optical tweezer slide 11.jpg]] | + | <p>[[File:optical tweezer slide 4.jpg|500px]]</p> |
| − | [[File:optical tweezer slide 12.jpg]] | + | Cut a short piece of double sided sticky tape. Cut this piece in half length-wise so you get two, skinny pieces of tape. |
| − | [[File:optical tweezer slide 13.jpg]] | + | |
| + | <p>[[File:optical tweezer slide 5.jpg|500px]]</p> | ||
| + | Put some vaseline on one side of both pieces of tape. | ||
| + | |||
| + | <p>[[File:optical tweezer slide 6.jpg|500px]]</p> | ||
| + | Place both of these pieces of tape on the microscope slide about a centimeter away from each other with the vaseline sides facing each other. | ||
| + | |||
| + | <p>[[File:optical tweezer slide 7.jpg|500px]]</p> | ||
| + | After cleaning a microscope cover slip in the same fashion as the slide, place a cover slip on top of the two pieces of tape. Be sure that the cover slip is secure. | ||
| + | |||
| + | <p>[[File:optical tweezer slide 8.jpg|500px]]</p> | ||
| + | Retrieve around 10 micro-liters of microsphere solution. Make sure that this is very dilute. If you can see any opacity, it is too concentrated. | ||
| + | |||
| + | <p>[[File:optical tweezer slide 9.jpg|500px]]</p> | ||
| + | |||
| + | |||
| + | <p>[[File:optical tweezer slide 10.jpg|500px]]</p> | ||
| + | Dry off any excess solution on the edges of the cover slip. | ||
| + | |||
| + | <p>[[File:optical tweezer slide 11.jpg|500px]]</p> | ||
| + | Use top coat nail polish to seal the ends of the channels. | ||
| + | |||
| + | <p>[[File:optical tweezer slide 12.jpg|500px]]</p> | ||
| + | |||
| + | <p>[[File:optical tweezer slide 13.jpg|500px]]</p> | ||
| + | Yay! | ||
Revision as of 14:04, 25 April 2014
Optical Tweezers
Here are some slide prepping instructions from Berkeley. [1]
Place lens tissue paper above and below a microscope slide.
To clean the slide, drop a small amount of isopropyl alcohol on top of the top piece of lens tissue.
Slide the lens tissue across the microscope slide, making sure that the alcohol wipes across the entire slide. You may need something to hold the slide in place to keep it from... well, sliding.
Cut a short piece of double sided sticky tape. Cut this piece in half length-wise so you get two, skinny pieces of tape.
Put some vaseline on one side of both pieces of tape.
Place both of these pieces of tape on the microscope slide about a centimeter away from each other with the vaseline sides facing each other.
After cleaning a microscope cover slip in the same fashion as the slide, place a cover slip on top of the two pieces of tape. Be sure that the cover slip is secure.
Retrieve around 10 micro-liters of microsphere solution. Make sure that this is very dilute. If you can see any opacity, it is too concentrated.
Dry off any excess solution on the edges of the cover slip.
Use top coat nail polish to seal the ends of the channels.
Yay!
