Difference between revisions of "Making Diluted Solutions of MicroSpheres"
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=== Procedure - From liquid with ~ 10% solids === | === Procedure - From liquid with ~ 10% solids === | ||
| − | * Shake microsphere bottle to evenly disperse the particles. | + | * Shake primary microsphere bottle to evenly disperse the particles. |
* Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution). | * Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution). | ||
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* For non-biological particles, 1% polyethylene glycol can be added to the microsphere solution as an anti-stick agent. | * For non-biological particles, 1% polyethylene glycol can be added to the microsphere solution as an anti-stick agent. | ||
| − | * For biological particles the BSA application and Phosphate-Buffered Saline (PBS - | + | * For biological particles the BSA application and Phosphate-Buffered Saline (PBS) (Sigma-Aldrich P5368-10PAK) can be used as anti-stick agents. |
| + | |||
| + | * If particles begin to stick to the slide surfaces, a 3 minute sonication can increase the mobility of particles. | ||
[http://en.wikipedia.org/wiki/Phosphate_buffered_saline PBS Wikipedia] | [http://en.wikipedia.org/wiki/Phosphate_buffered_saline PBS Wikipedia] | ||
''Students: If this recipe is inadequate (too many or too few particles) please revise. '' | ''Students: If this recipe is inadequate (too many or too few particles) please revise. '' | ||
Latest revision as of 17:19, 4 September 2015
Procedure - From liquid with ~ 10% solids
- Shake primary microsphere bottle to evenly disperse the particles.
- Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution).
Anti-Stick Agents
- Bovine Serum Albumin (BSA) at 20-25mg/ml in deionized water can be applied to the glass microscope slide (and coverslip) and allowed to evaporate off (to inhibit particle sticking to the glass surfaces).
- For non-biological particles, 1% polyethylene glycol can be added to the microsphere solution as an anti-stick agent.
- For biological particles the BSA application and Phosphate-Buffered Saline (PBS) (Sigma-Aldrich P5368-10PAK) can be used as anti-stick agents.
- If particles begin to stick to the slide surfaces, a 3 minute sonication can increase the mobility of particles.
Students: If this recipe is inadequate (too many or too few particles) please revise.
