Slide Set-Up - Revision history

Admin at 05:50, 5 September 2015

2015-09-05T05:50:51Z

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Wikiuser at 17:35, 23 January 2015

2015-01-23T17:35:12Z

Wikiuser at 22:43, 7 December 2014

2014-12-07T22:43:16Z

Wikiuser at 22:39, 7 December 2014

2014-12-07T22:39:29Z

Wikiuser at 22:35, 7 December 2014

2014-12-07T22:35:51Z

Wikiuser at 20:26, 24 November 2014

2014-11-24T20:26:01Z

Wikiuser at 19:48, 24 November 2014

2014-11-24T19:48:53Z

Wikiuser at 19:16, 24 November 2014

2014-11-24T19:16:47Z

Wikiuser at 19:15, 24 November 2014

2014-11-24T19:15:29Z

Wikiuser at 17:56, 21 August 2014

2014-08-21T17:56:12Z

@@ Line 1: / Line 1: @@
 Back to main [[Optical Tweezers]] Page.
 <table border="1">
      <tr>
@@ Line 54: / Line 57: @@
          </td>
          <td>
-             <p>After cleaning a microscope coverslip in the same fashion as the slide (and possibly coating with BSA), place a coverslip on top of the two pieces of tape.  Be sure that the cover slip is secure.</p>
+             <p>'''After cleaning a microscope coverslip in the same fashion as the slide''' (and possibly coating with BSA), place a coverslip on top of the two pieces of tape.  Be sure that the cover slip is secure.</p>
          </td>
      </tr>
@@ Line 105: / Line 108: @@
      </tr>
 </table>
 <h4>Important notes:</h4>

@@ Line 117: / Line 117: @@
      <li>
          To conserve microscope slides and to produce more stability, cleaving standard sized microscope slides into halves is a good idea.
      </li>
 </ul>

@@ Line 77: / Line 77: @@
          </td>
          <td>
-             <p>Dry off any excess solution on the edges of the cover slip.</p>
+             <p>Dry off any excess solution on the edges of the cover slip.  Cut away excess tape with an exacto knife or scalpel.</p>
          </td>
      </tr>
@@ Line 85: / Line 85: @@
          </td>
          <td>
-             <p>Use top coat nail polish to seal the ends of the channels.</p>
+             <p>Use top coat nail polish on all edges of the cover slip to seal it down.</p>
          </td>
      </tr>
@@ Line 93: / Line 93: @@
          </td>
          <td>
          </td>
      </tr>

@@ Line 38: / Line 38: @@
          </td>
          <td>
-             <p>Put some vaseline on one side of both pieces of tape.</p>
+             <p>Put some vaseline on one edge of both pieces of tape.  Make sure that you put on as little vaseline as possible while still coating the entire edge.</p>
          </td>
      </tr>

@@ Line 46: / Line 46: @@
          </td>
          <td>
-             <p>Place both of these pieces of tape on the microscope slide about a centimeter away from each other with the vaseline sides facing each other.</p>
+             <p>Place both of these pieces of tape on the microscope slide about a centimeter away from each other with the vaseline sides facing each other. Possibly coat (and let dry) the slide with a Bovine Serum Albumin (BSA) solution (to help prevent microspheres sticking irreversibly to the glass slide)</p>
          </td>
      </tr>
@@ Line 54: / Line 54: @@
          </td>
          <td>
-             <p>After cleaning a microscope cover slip in the same fashion as the slide, place a cover slip on top of the two pieces of tape.  Be sure that the cover slip is secure.</p>
+             <p>After cleaning a microscope coverslip in the same fashion as the slide (and possibly coating with BSA), place a coverslip on top of the two pieces of tape.  Be sure that the cover slip is secure.</p>
          </td>
      </tr>

@@ Line 62: / Line 62: @@
          </td>
          <td>
-             <p>Retrieve around 10 micro-liters of microsphere solution.  Make sure that this is very dilute.  If you can see any opacity, it is too concentrated.</p>
+             <p>Retrieve around 10 micro-liters of diluted microsphere solution.  Make sure that this is very dilute.  For example, if you have a starting liquid microsphere solution with 10% solids, shake the original bottle to evenly disperse the beads then try a dilution of ~ 200:1 of deionized water (you may need to dilute even more).  If you can see any opacity, it is too concentrated.</p>
          </td>
      </tr>
@@ Line 107: / Line 107: @@
 <h4>Important notes:</h4>
 <ul>
      <li>
-        Do not shake the contents of the microsphere bottles unless you plan to dilute them.  Trying to view this many spheres looks cool, but doesn't have any real value.  If you shake the bottle and then view the sample under the objective lens, you'll look like a fool.
+         Make sure to orient the slide so that the cover slip is facing towards the objective lens.  In our case, the working distance of our objective lens is 0.2 mm.  If your's is similar, the sample will be too far away to focus.
      </li>
      <li>

@@ Line 3: / Line 3: @@
      <tr>
          <td>
-             <p>1.[[File:optical tweezer slide 1.jpg|500px]]</p>
+             <p>1.[[File:optical tweezer slide 1.jpg|400px]]</p>
          </td>
          <td>
@@ Line 11: / Line 11: @@
      <tr>
          <td>
-             <p>2.[[File:optical tweezer slide 2.jpg|500px]]</p>
+             <p>2.[[File:optical tweezer slide 2.jpg|400px]]</p>
          </td>
          <td>
@@ Line 19: / Line 19: @@
      <tr>
          <td>
-             <p>3.[[File:optical tweezer slide 3.jpg|500px]]</p>
+             <p>3.[[File:optical tweezer slide 3.jpg|400px]]</p>
          </td>
          <td>
@@ Line 27: / Line 27: @@
      <tr>
          <td>
-             <p>4.[[File:optical tweezer slide 4.jpg|500px]]</p>
+             <p>4.[[File:optical tweezer slide 4.jpg|400px]]</p>
          </td>
          <td>
@@ Line 35: / Line 35: @@
      <tr>
          <td>
-             <p>5.[[File:optical tweezer slide 5.jpg|500px]]</p>
+             <p>5.[[File:optical tweezer slide 5.jpg|400px]]</p>
          </td>
          <td>
@@ Line 43: / Line 43: @@
      <tr>
          <td>
-             <p>6.[[File:optical tweezer slide 6.jpg|500px]]</p>
+             <p>6.[[File:optical tweezer slide 6.jpg|400px]]</p>
          </td>
          <td>
@@ Line 51: / Line 51: @@
      <tr>
          <td>
-             <p>7.[[File:optical tweezer slide 7.jpg|500px]]</p>
+             <p>7.[[File:optical tweezer slide 7.jpg|400px]]</p>
          </td>
          <td>
@@ Line 59: / Line 59: @@
      <tr>
          <td>
-             <p>8.[[File:optical tweezer slide 8.jpg|500px]]</p>
+             <p>8.[[File:optical tweezer slide 8.jpg|400px]]</p>
          </td>
          <td>
@@ Line 67: / Line 67: @@
      <tr>
          <td>
-             <p>9.[[File:optical tweezer slide 9.jpg|500px]]</p>
+             <p>9.[[File:optical tweezer slide 9.jpg|400px]]</p>
          </td>
          <td>
@@ Line 74: / Line 74: @@
      <tr>
          <td>
-              <p>10.[[File:optical tweezer slide 10.jpg|500px]]</p>
+              <p>10.[[File:optical tweezer slide 10.jpg|400px]]</p>
          </td>
          <td>
@@ Line 82: / Line 82: @@
      <tr>
          <td>
-             <p>11.[[File:optical tweezer slide 11.jpg|500px]]</p>
+             <p>11.[[File:optical tweezer slide 11.jpg|400px]]</p>
          </td>
          <td>
@@ Line 90: / Line 90: @@
      <tr>
          <td>
-             <p>12.[[File:optical tweezer slide 12.jpg|500px]]</p>
+             <p>12.[[File:optical tweezer slide 12.jpg|400px]]</p>
          </td>
          <td>
@@ Line 97: / Line 97: @@
      <tr>
          <td>
-             <p>13.[[File:optical tweezer slide 13.jpg|500px]]</p>
+             <p>13.[[File:optical tweezer slide 13.jpg|400px]]</p>
          </td>
          <td>

@@ Line 14: / Line 14: @@
          </td>
          <td>
-             <p>To clean the slide, drop a small amount of isopropyl alcohol on top of the top piece of lens tissue.</p>
+             <p>To clean the slide, drop a small amount of methanol on top of the top piece of lens tissue.</p>
          </td>
      </tr>

@@ Line 115: / Line 115: @@
      <li>
          Take special care when applying nail polish near the microscope slide edges. If there is nail polish where the slide contacts the grooves of the slide mount, the nail polish may cause jamming or dry inside the grooves.
      </li>
 </ul>