Slide Mount Apparatus - Revision history

Wikiuser: /* Microscope Slide Mount */

2014-12-10T21:38:26Z

‎Microscope Slide Mount

Wikiuser: /* Microscope Slide Mount */

2014-12-10T20:41:14Z

‎Microscope Slide Mount

Wikiuser: /* Microscope Slide Mount */

2014-08-24T04:20:55Z

‎Microscope Slide Mount

Wikiuser: /* Microscope Slide Mount */

2014-08-24T04:20:20Z

‎Microscope Slide Mount

Wikiuser: /* Microscope Slide Mount */

2014-08-24T04:18:59Z

‎Microscope Slide Mount

Wikiuser: /* Microscope Slide Mount */

2014-08-24T04:04:31Z

‎Microscope Slide Mount

Wikiuser: /* Microscope Slide Mount */

2014-08-24T04:02:43Z

‎Microscope Slide Mount

Wikiuser at 22:39, 21 August 2014

2014-08-21T22:39:03Z

Wikiuser at 19:40, 21 August 2014

2014-08-21T19:40:11Z

Wikiuser at 19:37, 21 August 2014

2014-08-21T19:37:28Z

@@ Line 18: / Line 18: @@
 The apparatus may be constructed in several different manors, but suggested method will now be described.
-First the translational stages are assembled.  We first added the piezo stages to Newport stage.  We suggest using the piezo stages as the x and y stages, and to orient the knobs up and to the right so as not to impede adjustment.  Then screw the aluminum base into the optics table, then screw the translational stages into the base.
+First the translational stages are assembled.  We first added the piezo stages to Newport stage.  We suggest using the piezo stages as the z and x stages, and to orient the knobs up and to the right, respectively, so as not to impede adjustment.  We attached the z stage first, as doing so allowed us to put the slide mount in a more advantageous position.  Then attach the slide mount to the last piezo stage, as doing so after it is already screwed into the base is difficult.  Then screw the aluminum base into the optics table, then screw the translational stages plus the slide mount into the base.  We suggest having the manual y axis screwed all the way out before doing this, so as to minimize the chance of bumping into the objective..  You should be very careful during this part because the translational stages cannot balance on their own, and may tip into the objective, damaging it.  We suggest having one lab partner hold the stage while the other screws it into the aluminum base.
 [[File:small.jpg]]

@@ Line 8: / Line 8: @@
 First a Newport ''462 Series'' three dimensional translation stage was obtained as a starting point for our slide apparatus. The adjustment knobs on this particular mount are good for larger scale adjustments but lack fine tuning capability. Additionally this mount alone cannot be electronically controlled. The Newport stage is the large silver stage in image below.
-Next, three Thorlabs ''NFL5DP20 5 mm piezo'' translation stages were obtained to modify the Newport stage and enable electronic control. Additionally, a Thorlabs 3-axis Piezo Controller, and several BNC cables are needed to drive the Thorlabs stages. Additionally a function generator will be needed to perform Stoke's drag force measurements.
+Next, two Thorlabs ''NFL5DP20 5 mm piezo'' translation stages were obtained to modify the Newport stage and enable electronic control. Additionally, a Thorlabs 3-axis Piezo Controller, and several BNC cables are needed to drive the Thorlabs stages. Additionally a function generator will be needed to perform Stoke's drag force measurements.
-Finally, the custom slide mount piece will need to be attached to the apparatus in order to actually mount the slide on the apparatus. This will be located on the lab bench, if the apparatus is not already set up.
+The translational stages are then all mounted on a custom aluminum base.
 '''Slide Mount Apparatus Construction'''
@@ Line 16: / Line 18: @@
 The apparatus may be constructed in several different manors, but suggested method will now be described.
-First, one of the Thorlabs translation stages was bolted to the table. Be sure to approximate the distance away from the objective needed before any construction occurs. Orient the adjustment knob facing out from the table. Next, Mount another Thorlabs stage with the adjustment knob oriented 90 degrees from the first stage. This will enable electronic control in both horizontal directions. Next, on the Newport three dimensional stage, mount the final Thorlabs stage and the slide mount onto the stage's vertical face. Finally mount the Newport stage onto the two Thorlabs stages already on the table. Orient the vertical Thorlabs stage and slide mount to face the microscope objective and adjust the position of the slide mount if necessary. An image of the final product is displayed below:
+First the translational stages are assembled.  We first added the piezo stages to Newport stage.  We suggest using the piezo stages as the x and y stages, and to orient the knobs up and to the right so as not to impede adjustment.  Then screw the aluminum base into the optics table, then screw the translational stages into the base.
 [[File:small.jpg]]

← Older revision		Revision as of 04:20, 24 August 2014
Line 20:		Line 20:
	[[File:small.jpg]]		[[File:small.jpg]]

−	'''Figure 1:''' Three Dimensional Piezo Controlled Microscope Slide Translation Mount	+	'''Figure 1:''' Three-Dimensional Piezo-Controlled Microscope Slide Translation Mount

← Older revision		Revision as of 04:20, 24 August 2014
Line 19:		Line 19:

	[[File:small.jpg]]		[[File:small.jpg]]
		+
		+	'''Figure 1:''' Three Dimensional Piezo Controlled Microscope Slide Translation Mount

@@ Line 2: / Line 2: @@
 == '''Microscope Slide Mount''' ==
-For several reasons, the microscope slide mount is important piece of the optical tweezers set up. First, it needs to be sturdy enough to damp vibrations that move through the table and the lab. Since the Brownian motion measurements are observing particle oscillations of fractions of micrometers, any instability in the slide mount will skew the data. Secondly, it needs to be adjustable in all three dimensions focus the sample, and scan over the slide surface. Thirdly, the mount must be electronically controlled in the horizontal directions to perform Stokes measurements. Ideally it will be electronically controlled in the vertical direction to finely focus the objective, without having to touch the slide mount.
+For several reasons, the microscope slide mount is an important piece of the optical tweezers set up. First, it needs to be sturdy enough to damp vibrations that move through the table and the lab. Since the Brownian motion measurements are observing particle oscillations of fractions of micrometers, any instability in the slide mount will skew the data. Secondly, it needs to be adjustable in all three dimensions to focus the sample, and scan over the slide surface. Thirdly, the mount must be electronically controlled in the horizontal directions to perform Stokes measurements. Ideally it will be electronically controlled in the vertical direction to finely focus the objective, without having to touch the slide mount. Touching the mount actually alter the focus of the objective.
 '''Slide Mount Apparatus Components'''
-First a Newport ''462 Series'' three dimensional translation stage was obtained as a starting point for our slide apparatus. The adjustment knobs on this particular mount are good for larger scale adjustments but lack fine tuning capability. Additionally this mount alone cannot be electronically controlled. The Newport translation stage is displayed below.
+First a Newport ''462 Series'' three dimensional translation stage was obtained as a starting point for our slide apparatus. The adjustment knobs on this particular mount are good for larger scale adjustments but lack fine tuning capability. Additionally this mount alone cannot be electronically controlled. The Newport stage is the large silver stage in image below.
-Next, three Thorlabs ''NFL5DP20 5 mm piezo'' translation stages were obtained to modify the Newport stage and enable electronic control. Additionally, a Thorlabs 3-axis Piezo Controller, and several BNC cables are needed to drive the Thorlabs stages.
+Next, three Thorlabs ''NFL5DP20 5 mm piezo'' translation stages were obtained to modify the Newport stage and enable electronic control. Additionally, a Thorlabs 3-axis Piezo Controller, and several BNC cables are needed to drive the Thorlabs stages. Additionally a function generator will be needed to perform Stoke's drag force measurements.
-Finally, the custom slide mount piece will need to be attached to the apparatus in order to actually mount the slide on the apparatus.
+Finally, the custom slide mount piece will need to be attached to the apparatus in order to actually mount the slide on the apparatus. This will be located on the lab bench, if the apparatus is not already set up.
 '''Slide Mount Apparatus Construction'''
@@ Line 23: / Line 23: @@
 '''Using Slide Mount Apparatus'''
-A few tips for using this apparatus effectively are: Use the Newport adjustment knobs for large adjustments, save the Thorlabs stages for finer tuning. Always have the vertical Thorlabs stage connected to the piezo controller. This will make focusing the laser on the cover slide, much easier as you won't have to touch the apparatus at all. Finally, BE CAREFUL when adjusting the slide vertically. If the slide is pressed too hard against the microscope objective, the coverslip will crack, which is dangerous to the microscope objective.
+A few tips for using this apparatus effectively are: Use the Newport adjustment knobs for large adjustments, save the Thorlabs stages for finer tuning. Think about the starting position of each adjustment knob. Try not to max out either of the directions before adjusting the slide's position. Always have the vertical Thorlabs stage connected to the piezo controller. This will make focusing the laser on the cover slide, much easier as you won't have to touch the apparatus at all. Finally, BE CAREFUL when adjusting the slide vertically. If the slide is pressed too hard against the microscope objective, the cover-slip will crack, which is dangerous to the microscope objective.