Optical Tweezers - Revision history

Bsboggs: /* Optical Tweezers */

2018-05-07T19:45:51Z

‎Optical Tweezers

2016-10-27T17:36:13Z

2016-10-27T17:34:59Z

2016-10-27T16:58:12Z

2016-10-27T16:43:26Z

2016-10-27T16:34:16Z

2015-09-05T06:07:45Z

2015-09-05T01:28:38Z

2015-03-16T22:25:20Z

‎Optical Tweezers

2014-12-10T20:32:47Z

‎Optical Tweezers

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-        First remove the slide so that you have only the objective, then position a pair of pinholes straight above the objective, and adjust the objective and mirrors until the laser can shoot through both pinholes.  That's the rough calibration.
+        First remove the slide and the objective, then position a pair of (0.5 - 0.75mm) pinholes straight above the objective, and adjust the mirrors until the laser can shoot through both pinholes. That's the rough calibration.
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-        Next, put the slide on and and adjust the focus until you can see the interference patterns the laser makes on the slide.  Then adjust the mirrors until the interference patterns are are round.  Then the laser should be pointed straight at the slide.
+        Next, put the slide and objective back on and adjust the z-axis until you can see the interference patterns the laser makes on the slide.  Then adjust (rotate) the objective until the interference patterns are round. Then the laser should be pointed straight at the slide.
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-        Summer 2016 Powerpoint[http://hank.uoregon.edu/wiki/index.php/File:Optical_Tweezers_2016.pdf]
+        Summer 2016 Powerpoint[http://hank.uoregon.edu/wiki/images/a/a0/Optical_Tweezers_2016.pdf]
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 <h3>Vortex Wave Retarder</h3>
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          <li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li>
 </ul>
+</ul>
 <h3>Vortex Wave Retarder</h3>
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          <li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li>
 </ul>
-</li>
 <h3>Vortex Wave Retarder</h3>
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 <h3>Our own setup</h3>
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-     <li>
+     <li> '''Objective lens alignment & care'''
          Slide Setup
          <ul>

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 The pattern matching does work quite well for the purposes of tracking brownian motion.  You need to create a pattern matching script and then run it through batch processing.
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 We actually tried both using pattern matching and brute force point and click methods.  Neither worked very well, so we recommend using the QPD to get position measurements.  You don't need an actual video for this.
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-         [http://www.instructables.com/id/Image-Acquisition-in-NI-Vision-Assistant/] Image Acquisition/Saving Images
+         [http://www.instructables.com/id/Image-Acquisition-in-NI-Vision-Assistant/] Image Acquisition/Saving Images.  The UI for this program is rather difficult.  To exit from video capture mode after capturing images there is a button that says close near the bottom left, underneath the camera options window.
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-             We actually tried both using pattern matching and brute force point and click methods.  Neither worked very well, so we recommend using the QPD to get position measurements
+             To get an actual video, you need to run the pictures through another video editing program.  There is one on the computer, but there is no video option in NI Vision Assistant itself.
          </li>
      </ul>